Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Neural
Cell type
Anterior temporal cortex
NA
NA

Attributes by original data submitter

Sample

source_name
Adult anterior temporal cortex
developmental stage
Adult
tissue
brain (adult anterior temporal cortex)
chip antibody
CTCF (Abcam, ab70303)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, nuclei were extracted and chromatins were fragmented by sonication. The TF/histone-DNA complexes were isolated by antibody. All ChIP-seq libraries were generated following a ChIPmentation protocol with Nextera adapters.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
20736910
Reads aligned (%)
62.9
Duplicates removed (%)
35.5
Number of peaks
2892 (qval < 1E-05)

hg38

Number of total reads
20736910
Reads aligned (%)
65.0
Duplicates removed (%)
34.0
Number of peaks
2881 (qval < 1E-05)

Base call quality data from DBCLS SRA