GSM3673604: Input-ChIP-PS1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HUVEC
flowpattern
pulsatile shear
antibody
Input
assay
ChIP
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed at 37°C with 1% paraformaldehyde for 15 min, washed twice in cold phosphate buffered saline and scraped and pelleted at 3000 rpm for 5 min; cell pellets were resuspended in 50 uL lysis buffer, then diluted with 500 uL TE buffer for sonication. 20 ug chromatin, 3 ug antibody, and 11 uL dynabeads were incubated in ChIP buffer at 4°C overnight. The chromatin were eluted by 150 uL elution buffer followed by decrosslinking at 65℃ overnight. After incubation with RNase A and Proteinase K, DNA was precipitated by phenol:chloroform:isoamyl (25:24:1, v/v), dissolved in TE buffer, and stored at -80°C. Librariy preparation and Illumina HiSeq 2000 sequencing were done by the IGM Genomics Core, University of California, San Diego.