Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HUVEC
flowpattern
oscillatory shear
antibody
H3K27ac
assay
ChIP

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed at 37°C with 1% paraformaldehyde for 15 min, washed twice in cold phosphate buffered saline and scraped and pelleted at 3000 rpm for 5 min; cell pellets were resuspended in 50 uL lysis buffer, then diluted with 500 uL TE buffer for sonication. 20 ug chromatin, 3 ug antibody, and 11 uL dynabeads were incubated in ChIP buffer at 4°C overnight. The chromatin were eluted by 150 uL elution buffer followed by decrosslinking at 65℃ overnight. After incubation with RNase A and Proteinase K, DNA was precipitated by phenol:chloroform:isoamyl (25:24:1, v/v), dissolved in TE buffer, and stored at -80°C. Librariy preparation and Illumina HiSeq 2000 sequencing were done by the IGM Genomics Core, University of California, San Diego.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
30887414
Reads aligned (%)
99.6
Duplicates removed (%)
8.4
Number of peaks
61425 (qval < 1E-05)

hg19

Number of total reads
30887414
Reads aligned (%)
99.3
Duplicates removed (%)
8.6
Number of peaks
61358 (qval < 1E-05)

Base call quality data from DBCLS SRA