Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ERG

Cell type

Cell type Class
Cardiovascular
Cell type
HAEC
Primary Tissue
Aorta
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
arterial endothelial cells
cell type
HUAEC
passage
3
genotype
wildtype
chip antibody
ERG (Abcam, catalog number 92513)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, DNA was extracted using phenol-chloroform followed by ethanol precipitation and quantified using Qubit Fluorometric Quantitation assay ChIP-Seq libraries were constructed using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (New England Biolabs) with 50-100 ng of ChIP DNA according to manufacturer's protocol. In all cases, library quality and concentration were determined using an Agilent bioanalyzer (UMass Medical School Deep Sequencing Core)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
51345441
Reads aligned (%)
98.8
Duplicates removed (%)
6.3
Number of peaks
8831 (qval < 1E-05)

hg19

Number of total reads
51345441
Reads aligned (%)
97.8
Duplicates removed (%)
7.8
Number of peaks
8114 (qval < 1E-05)

Base call quality data from DBCLS SRA