Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR2F2

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
venous endothelial cells
cell type
HUVEC
passage
3
genotype
wildtype
chip antibody
NR2F2 (Perseus Proteomics, catalog number PP-H7147-00)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, DNA was extracted using phenol-chloroform followed by ethanol precipitation and quantified using Qubit Fluorometric Quantitation assay ChIP-Seq libraries were constructed using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (New England Biolabs) with 50-100 ng of ChIP DNA according to manufacturer's protocol. In all cases, library quality and concentration were determined using an Agilent bioanalyzer (UMass Medical School Deep Sequencing Core)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
3917022
Reads aligned (%)
96.2
Duplicates removed (%)
1.7
Number of peaks
155 (qval < 1E-05)

hg38

Number of total reads
3917022
Reads aligned (%)
97.7
Duplicates removed (%)
0.8
Number of peaks
133 (qval < 1E-05)

Base call quality data from DBCLS SRA