GSM3673432: HUVEC H3K27me3 ChIPSeq Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
venous endothelial cells
cell type
HUVEC
passage
3
genotype
wildtype
chip antibody
H3K27me3 (Active Motif, catalog number 39156)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, DNA was extracted using phenol-chloroform followed by ethanol precipitation and quantified using Qubit Fluorometric Quantitation assay ChIP-Seq libraries were constructed using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (New England Biolabs) with 50-100 ng of ChIP DNA according to manufacturer's protocol. In all cases, library quality and concentration were determined using an Agilent bioanalyzer (UMass Medical School Deep Sequencing Core)