Antigen

Antigen Class

TFs and others

Antigen

EP300

Cell type

Cell type Class

Cardiovascular

Cell type

HUVEC

Primary Tissue

Umbilical Cord

Tissue Diagnosis

Normal

Sample

source_name

venous endothelial cells

cell type

HUVEC

passage

3

genotype

wildtype

chip antibody

EP300 (Cell Signaling, catalog number 4771)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, DNA was extracted using phenol-chloroform followed by ethanol precipitation and quantified using Qubit Fluorometric Quantitation assay ChIP-Seq libraries were constructed using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (New England Biolabs) with 50-100 ng of ChIP DNA according to manufacturer's protocol. In all cases, library quality and concentration were determined using an Agilent bioanalyzer (UMass Medical School Deep Sequencing Core)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

38275926

Reads aligned (%)

99.4

Duplicates removed (%)

8.2

Number of peaks

46755 (qval < 1E-05)

hg19

Number of total reads

38275926

Reads aligned (%)

98.8

Duplicates removed (%)

8.9

Number of peaks

46657 (qval < 1E-05)