GSM3669470: Δ CTRL Input (3); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Neural
Cell type
LUHMES
Tissue
Mesencephalon
Attributes by original data submitter
Sample
source_name
LUHMES
infection
HSV-1 DCTRL2
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
TGs were homogenized, crosslinked in 1% formaldehyde, and the cell lysates were sonicated. The sheared chromatin was pre-cleared with salmon-sperm DNA-protein A-agarose beads prior to antibody incubation. An aliquot representing 1/5 of the total sample volume was removed as a sample input (I). Both bound and input fractions were treated with 5M NaCl, RNAse A and proteinase K and the DNA was finally purified using a Qiaquick PCR purification kit. Prior to Illumina library construction and hybridization capture-based target enrichment. Briefly, 8 PCR-cycle indexed libraries were pooled for selection using the SureSelect XT hs system with custom probes spanning the HSV-1 genome (Roche). Samples were subject to 4 additional PCR cycles and used for 100 base pair paired-end Illumina sequencing on the HiSeq platform. Kappa biosystems hyperprep