Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
MEFs-Input-NC-ChIPseq
cell type
MEF cell line
passages
6~10
treated with
negative control siRNA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
MEFs were fixed with 1% Formaldehyde at room temperature for 10 min. After the treatment, cross linking was quenched with 0.125 M glycine for 10 min. ~50 million cells were used for each ChIP.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
30962288
Reads aligned (%)
94.0
Duplicates removed (%)
13.4
Number of peaks
12438 (qval < 1E-05)

mm9

Number of total reads
30962288
Reads aligned (%)
93.8
Duplicates removed (%)
13.4
Number of peaks
12418 (qval < 1E-05)

Base call quality data from DBCLS SRA