Antigen

Antigen Class

TFs and others

Antigen

HIRA

Cell type

Cell type Class

Unclassified

Cell type

Unclassified

NA

NA

Sample

source_name

shPML.IFN-Pos

genotype/variation

Short-hairpin targeting PML

treatment

Interferon Stimuated

chip antibody

mouse mAbs to HIRA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fragmented chromatin was incubated with antibodies to HIRA and preimmobilized on Dynabeads. Libraries were prepared from 10–20 ng of ChIP or input DNA using New England Biolabs ChIP-seq kit according to the manufacturer's instructions Resulting libraries were sequenced on an Illumina GAIIx to yield ∼30 million to 40 million raw reads.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

35000925

Reads aligned (%)

93.3

Duplicates removed (%)

15.0

Number of peaks

2573 (qval < 1E-05)

hg19

Number of total reads

35000925

Reads aligned (%)

90.2

Duplicates removed (%)

21.3

Number of peaks

1717 (qval < 1E-05)