GSM3666133: shCntrl.IFN-Neg.ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
HIRA
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
shCntrl.IFN-Neg
genotype/variation
Short-hairpin control
treatment
Control
chip antibody
mouse mAbs to HIRA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fragmented chromatin was incubated with antibodies to HIRA and preimmobilized on Dynabeads. Libraries were prepared from 10–20 ng of ChIP or input DNA using New England Biolabs ChIP-seq kit according to the manufacturer's instructions Resulting libraries were sequenced on an Illumina GAIIx to yield ∼30 million to 40 million raw reads.