GSM3659525: LOUCY KPT-330 anti-Nup214 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NUP214
Cell type
Cell type Class
Blood
Cell type
Loucy
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
LOUCY
cell type
human leukemia
cell line
LOUCY
chip antibody
anti-Nup214(Bethyl Laboratories, A300-716A)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cross-linked chromatin was sheared by sonication, and MNase treatment. To repair ChIP-DNA and Input-DNA ends, T4 DNA polymerase, Klenow enzyme and T4 polynucleotide kinase were used (New England Biolabs), and an A base was subsequently added to the 3'-end by the treatment of Klenow exo-. The adaptor ligation and the PCR amplification for 18 cycles of DNA fragments were performed with Illumina (PCR primer InPE1.0 & InPE2.0).