Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
XPO1

Cell type

Cell type Class
Blood
Cell type
Loucy
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
LOUCY
cell type
human leukemia
cell line
LOUCY
chip antibody
anti-Crm1 (CST, 46249)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cross-linked chromatin was sheared by sonication, and MNase treatment. To repair ChIP-DNA and Input-DNA ends, T4 DNA polymerase, Klenow enzyme and T4 polynucleotide kinase were used (New England Biolabs), and an A base was subsequently added to the 3'-end by the treatment of Klenow exo-. The adaptor ligation and the PCR amplification for 18 cycles of DNA fragments were performed with Illumina (PCR primer InPE1.0 & InPE2.0).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
12790709
Reads aligned (%)
78.6
Duplicates removed (%)
19.8
Number of peaks
223 (qval < 1E-05)

hg38

Number of total reads
12790709
Reads aligned (%)
79.6
Duplicates removed (%)
19.2
Number of peaks
210 (qval < 1E-05)

Base call quality data from DBCLS SRA