Antigen

Antigen Class

TFs and others

Antigen

XPO1

Cell type

Cell type Class

Blood

Cell type

OCI-AML3

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

OCI-AML3

cell type

human leukemia

cell line

OCI-AML3

chip antibody

anti-Crm1 (CST, 46249)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The cross-linked chromatin was sheared by sonication, and MNase treatment. To repair ChIP-DNA and Input-DNA ends, T4 DNA polymerase, Klenow enzyme and T4 polynucleotide kinase were used (New England Biolabs), and an A base was subsequently added to the 3'-end by the treatment of Klenow exo-. The adaptor ligation and the PCR amplification for 18 cycles of DNA fragments were performed with Illumina (PCR primer InPE1.0 & InPE2.0).

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg38

Number of total reads

14892203

Reads aligned (%)

83.4

Duplicates removed (%)

76.1

Number of peaks

62 (qval < 1E-05)

hg19

Number of total reads

14892203

Reads aligned (%)

82.9

Duplicates removed (%)

77.2

Number of peaks

82 (qval < 1E-05)