Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Primary B-cells
strain
C57BL/6
cell type
B cells from spleen
genotype/variation
WT
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 250 bp were immunoprecipitated using different antibodies. Libraries were prepared for Illumina sequencing using a standard protocol consisting in blunting, addition of dA overhangs, ligation of Illumina adapters, selection on gel and PCR with index primers.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
15239014
Reads aligned (%)
93.1
Duplicates removed (%)
10.8
Number of peaks
1089 (qval < 1E-05)

mm9

Number of total reads
15239014
Reads aligned (%)
92.8
Duplicates removed (%)
10.9
Number of peaks
1157 (qval < 1E-05)

Base call quality data from DBCLS SRA