Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
Th17 Cells
MeSH Description
A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Attributes by original data submitter

Sample

source_name
Th17
treatment
GSK-J4
donor
3
chip antibody
H3K4me3
cell type
Th17 cell

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
12424805
Reads aligned (%)
97.0
Duplicates removed (%)
1.9
Number of peaks
7206 (qval < 1E-05)

hg19

Number of total reads
12424805
Reads aligned (%)
96.2
Duplicates removed (%)
2.9
Number of peaks
7263 (qval < 1E-05)

Base call quality data from DBCLS SRA