Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
Th17 Cells
MeSH Description
A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Attributes by original data submitter

Sample

source_name
Th17
treatment
GSK-J4
donor
2
chip antibody
H3K4me3
cell type
Th17 cell

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
5062779
Reads aligned (%)
97.8
Duplicates removed (%)
4.3
Number of peaks
11764 (qval < 1E-05)

hg19

Number of total reads
5062779
Reads aligned (%)
97.0
Duplicates removed (%)
5.2
Number of peaks
11804 (qval < 1E-05)

Base call quality data from DBCLS SRA