Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Th17 Cells

MeSH Description

A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Sample

source_name

Th17

treatment

GSK-J4

donor

1

chip antibody

H3K4me3

cell type

Th17 cell

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

4959741

Reads aligned (%)

98.7

Duplicates removed (%)

4.2

Number of peaks

11675 (qval < 1E-05)

hg19

Number of total reads

4959741

Reads aligned (%)

97.9

Duplicates removed (%)

5.1

Number of peaks

11682 (qval < 1E-05)