Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Placenta

Cell type

Trophoblasts

MeSH Description

Trophoblasts with multinuclear cytoplasm and undefined cellular structures. Syncytiotrophoblasts secrete proteolytic enzymes that erode the ENDOMETRIUM for EMBRYO IMPLANTATION. They also produce steroid, peptide, and protein hormones (PLACENTAL HORMONES).

Sample

source_name

trophoblast primary culture

tissue

Mid-gestation placenta

chip antibody

H3K4me3 (39159, Active Motif)

shearing

Mnase

cell culture

trophoblast primary culture

treatment

Synotrophoblast (18h)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Trophoblasts were crosslinked with 1% formaldehyd and then either treated with Mnase or sheared to 200-500 bp by ultrasonication. For both procedures, chromatin was isolated and used for ChIP according to standard procedures. Libraries were constructed according to the Illumina Tru-seq protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

46916667

Reads aligned (%)

95.6

Duplicates removed (%)

24.7

Number of peaks

43733 (qval < 1E-05)

hg19

Number of total reads

46916667

Reads aligned (%)

95.0

Duplicates removed (%)

25.7

Number of peaks

43387 (qval < 1E-05)