Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXP1

Cell type

Cell type Class
Blood
Cell type
OCI-LY1
NA
NA

Attributes by original data submitter

Sample

source_name
OCI-LY1 cells
tissue
OCI-LY7 cells
chip antibody
FOXP1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sheared to 200-400bp, incubated with H3K27ac antibody (abcam 4729) overnight at 4C on a rocker, antibody chromatin complex was captured by ProteinA agarose beads, washed with low salt, high salt, LiCl, and TE buffer, eluted and then de-crosslinked at 65C with 0.3M NaCl final for at least 6 hr. Final chormatin was purified using Qiagen PCR purification columns. Libraries were prepared using the Illumina Truseq ChIPseq Library preparation Kit following the manufacture's instructions (Illumina, CA).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
278426200
Reads aligned (%)
54.9
Duplicates removed (%)
21.3
Number of peaks
117444 (qval < 1E-05)

hg38

Number of total reads
278426200
Reads aligned (%)
56.1
Duplicates removed (%)
20.2
Number of peaks
119135 (qval < 1E-05)

Base call quality data from DBCLS SRA