Antigen

Antigen Class

TFs and others

Antigen

FOXP1

Cell type

Cell type Class

Blood

Cell type

OCI-LY1

NA

NA

Sample

source_name

OCI-LY1 cells

tissue

OCI-LY7 cells

chip antibody

FOXP1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was sheared to 200-400bp, incubated with H3K27ac antibody (abcam 4729) overnight at 4C on a rocker, antibody chromatin complex was captured by ProteinA agarose beads, washed with low salt, high salt, LiCl, and TE buffer, eluted and then de-crosslinked at 65C with 0.3M NaCl final for at least 6 hr. Final chormatin was purified using Qiagen PCR purification columns. Libraries were prepared using the Illumina Truseq ChIPseq Library preparation Kit following the manufacture's instructions (Illumina, CA).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

278426200

Reads aligned (%)

70.3

Duplicates removed (%)

20.2

Number of peaks

119135 (qval < 1E-05)

hg19

Number of total reads

278426200

Reads aligned (%)

69.8

Duplicates removed (%)

21.3

Number of peaks

117444 (qval < 1E-05)