Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BigH1

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
wt S2 cells
cell line
S2 cells
genotype/variation
wt
type
IP dBigH1
replicate
2
chip antibody
polyclonal αdBigH1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with polyclonal αdBigH1 and αH1 antibodies. Finally DNA was purified by standard phenol extraction. 10 ng DNA, quantified by Qubit dsDNA HS Assay Kit (Invitrogen) was used for library preparation. Endrepair, adenylation, ligation of adapters and PCR enrichment for 10 cycles was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer's recommendations. Purified libraries were quantified by Qubit dsDNA HS Assay Kit (Invitrogen) and size distribution was evaluated using Bioanalyzer DNA 1000 assay (Agilent).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
46698560
Reads aligned (%)
76.2
Duplicates removed (%)
35.3
Number of peaks
5899 (qval < 1E-05)

dm6

Number of total reads
46698560
Reads aligned (%)
75.2
Duplicates removed (%)
36.8
Number of peaks
5197 (qval < 1E-05)

Base call quality data from DBCLS SRA