GSM1385968: dmCas9-ChIPseq No sgRNA WCE; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast
Attributes by original data submitter
Sample
source_name
dmCas9-ChIPseq No sgRNA WCE
cell line
Arf −/− MEFs
cell type
mouse embryonic fibroblasts
antibody
No antibody
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Protein-DNA complexes were immunoprecipitated from sonicated chromatin extract with anti-FLAG antibody. NEBNext ChIP-Seq Library Prep Reagent Set for Illumina (E6200S, NEB) according to the manufacturer's protocol. For BARCODE ChIP-seq library we used the BARCODE adaptor for generating the library (BARCODE adaptor: PMID 19159457).