Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9ac

Cell type

Cell type Class
Digestive tract
Cell type
TE7
Primary Tissue
Esophagus
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
esophagus
cell line
TE-7
cell type
esophageal epithelial
chip antibody
ab10812 (Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP cells were fixed with 0.8% formaldehyde for 8 min at RT and DNA was processed and sonicated according to the protocol from Covaris using Covaris S220 sonicator. RNA and DNA libraries for single read sequencing were prepared for using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
11096060
Reads aligned (%)
96.8
Duplicates removed (%)
51.9
Number of peaks
13361 (qval < 1E-05)

hg19

Number of total reads
11096060
Reads aligned (%)
96.2
Duplicates removed (%)
52.7
Number of peaks
13364 (qval < 1E-05)

Base call quality data from DBCLS SRA