Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NETO2

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
human AML cells
cell line
Kasumi-1
cell type
human t(8;21) AML cells
chip antibody
ETO2 (Santa Cruz, SC-9741)
transduced with
lentiviruses expressing ETO2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using SimpleChIP® Enzymatic Chromatin IP Kits (Magnetic Beads, Cell Signaling Technology #9003) following the manufacturer's instructions. Libraries were constructed following instructions accompanying the library preparation kit designed for Illumina sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
105171443
Reads aligned (%)
49.5
Duplicates removed (%)
49.2
Number of peaks
17644 (qval < 1E-05)

hg38

Number of total reads
105171443
Reads aligned (%)
51.1
Duplicates removed (%)
48.0
Number of peaks
18140 (qval < 1E-05)

Base call quality data from DBCLS SRA