Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NETO2

Cell type

Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
human normal CD34+ HSPCs
cell type
human CD34+ HSPCs
chip antibody
ETO2 (Santa Cruz, SC-9741)
transduced with
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using SimpleChIP® Enzymatic Chromatin IP Kits (Magnetic Beads, Cell Signaling Technology #9003) following the manufacturer's instructions. Libraries were constructed following instructions accompanying the library preparation kit designed for Illumina sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
83970866
Reads aligned (%)
175.8
Duplicates removed (%)
9.6
Number of peaks
63480 (qval < 1E-05)

hg38

Number of total reads
83970866
Reads aligned (%)
176.4
Duplicates removed (%)
9.5
Number of peaks
64394 (qval < 1E-05)

Base call quality data from DBCLS SRA