Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PGR

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D A1/2 cells
cell type
Mammary epithelial cells
cell line
T47D A1/2 cells
chip antibody
anti-PR, H-190, sc-7208, Santa Cruz Biotechnology
agent
R5020 (10 nM)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
57028133
Reads aligned (%)
96.4
Duplicates removed (%)
9.6
Number of peaks
27129 (qval < 1E-05)

hg19

Number of total reads
57028133
Reads aligned (%)
95.7
Duplicates removed (%)
10.7
Number of peaks
26960 (qval < 1E-05)

Base call quality data from DBCLS SRA