Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D A1/2 cells
cell type
Mammary epithelial cells
cell line
T47D A1/2 cells
chip antibody
Mix: anti-GR, P-20, sc-1002, Santa Cruz Biotechnology and anti-GR, PA1-511A, Thermo Fisher Scientific
agent
R5020 (10 nM)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
173831017
Reads aligned (%)
61.2
Duplicates removed (%)
36.6
Number of peaks
2323 (qval < 1E-05)

hg38

Number of total reads
173831017
Reads aligned (%)
63.6
Duplicates removed (%)
34.7
Number of peaks
3084 (qval < 1E-05)

Base call quality data from DBCLS SRA