Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MLXIP

Cell type

Cell type Class
Muscle
Cell type
Skeletal myotubes
NA
NA

Attributes by original data submitter

Sample

source_name
Primary skeletal myotube
cell type
Primary human myocytes
genotype
MondoA KD
passages
P7
chip antibody
MondoA (ProteinTech, catalog # 13614-1-AP, lot # 00042515)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and MondoA or Histione-DNA complexes were isolated with antibody. DNA libraries were prepared for sequencing using Ovation® Ultralow V2 DNA-Seq Library Preparation Kit (NuGEN, Catalog # 0344)

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
73827420
Reads aligned (%)
56.5
Duplicates removed (%)
26.9
Number of peaks
1158 (qval < 1E-05)

hg38

Number of total reads
73827420
Reads aligned (%)
57.6
Duplicates removed (%)
26.0
Number of peaks
1038 (qval < 1E-05)

Base call quality data from DBCLS SRA