Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Neural
Cell type
Oligodendrocytes
NA
NA

Attributes by original data submitter

Sample

source_name
differentiated oligodendrocyte
strain
FvB/C57BL6 hybrid
cell type
differentiated oligodendrocyte from neural stem cell with ectopic expression of PPARb
passages
7
chip antibody
RNA polymerase II CTD repeat YSPTSPS (Abcam, Cat# ab817)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complex were isolated with Qki-5, PPARb, HA, and Pol II antibodies. Libraries were prepared by VAHTS Universal DNA Library Prep Kit (Vazyme, ND607)

Sequencing Platform

instrument_model
HiSeq X Ten

mm10

Number of total reads
52718336
Reads aligned (%)
62.0
Duplicates removed (%)
20.5
Number of peaks
2618 (qval < 1E-05)

mm9

Number of total reads
52718336
Reads aligned (%)
61.9
Duplicates removed (%)
20.4
Number of peaks
2588 (qval < 1E-05)

Base call quality data from DBCLS SRA