Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pancreas
Cell type
INS-1E
Primary Tissue
Pancreas
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
H3AcK27_ChIPseq
cell line
INS-1
cell type
insulinoma cells
sample treatment
Ad-GFP/1 hour 10 uM forskolin
chip antibody
rabbit anti-H3AcK27 (Abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody ChIP-seq libraries were constructed with NEBNext ChIPseq library kit (NEB) according to manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

rn6

Number of total reads
22267420
Reads aligned (%)
96.6
Duplicates removed (%)
21.6
Number of peaks
41360 (qval < 1E-05)

Base call quality data from DBCLS SRA