Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RORC

Cell type

Cell type Class
Breast
Cell type
HCC70
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
triple-negative breast cancer cells
cell line
HCC70
passages
6-9
treatment
vehicle
chip antibody
anti-RORγ rabbit serum was generated by Covance, using purified GST-human RORγ fragment (amino acids 79-301) expressed in Escherichia coli.

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's paired-end DNA sample Prep kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
23615089
Reads aligned (%)
73.3
Duplicates removed (%)
21.6
Number of peaks
23745 (qval < 1E-05)

hg38

Number of total reads
23615089
Reads aligned (%)
78.3
Duplicates removed (%)
17.3
Number of peaks
24305 (qval < 1E-05)

Base call quality data from DBCLS SRA