Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
KG-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
KG1 cell line
tissue
bone marrow
cell line
KG3
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA or proteins-DNA complexes were isolated with antibody. DNA was purified using the i-Pure kit (Diagenode) Libraries were prepared according to Illumina's instructions accompanying the Illumina's TruSeq ChIP sample Prep Kit Protocol

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
22818690
Reads aligned (%)
97.7
Duplicates removed (%)
26.1
Number of peaks
683 (qval < 1E-05)

hg19

Number of total reads
22818690
Reads aligned (%)
96.8
Duplicates removed (%)
26.7
Number of peaks
383 (qval < 1E-05)

Base call quality data from DBCLS SRA