Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Blood
Cell type
KG-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
KG1 cell line
tissue
bone marrow
cell line
KG2
antibody
H3K4me1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA or proteins-DNA complexes were isolated with antibody. DNA was purified using the i-Pure kit (Diagenode) Libraries were prepared according to Illumina's instructions accompanying the Illumina's TruSeq ChIP sample Prep Kit Protocol

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
29850551
Reads aligned (%)
97.8
Duplicates removed (%)
65.8
Number of peaks
841 (qval < 1E-05)

hg19

Number of total reads
29850551
Reads aligned (%)
97.3
Duplicates removed (%)
66.3
Number of peaks
718 (qval < 1E-05)

Base call quality data from DBCLS SRA