Total RNA was isolated from cell pellets of WT (Lsh+/+) MEFs and KO (Lsh-/-) MEFs and their biologic replica using the RNeasy Mini Kit (Qiagen) and treated with DNaseI (Roche) for 10 min at room temperature. rRNAs were removed from 5 μg of total RNA by RiboMinus (Life Technologies) as per the manufacturer’s instructions. Cells were cross-linked with formaldehyde and nuclear DNA was sonicated with 200-300bp, then the H3K4me1-DNA complexes were isolated with antibody. The poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. After purification and PCR amplification the final cDNA library was generated based on the mRNA-seq Library Preparation Protocol from Illumina. ChIP DNA libraries were made following Illumina ChIP-Seq library preparation kit and subjected to Solexa sequencing (Illumina) at the CCR-Sequencing Facility, National Cancer Institute. Sequencing was performed on Illumina Genome Analyzer IIx.