Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
HCT116 WT
cell type
human colon cancer cells
cell line
HCT116
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Five million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1 Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
19010646
Reads aligned (%)
98.1
Duplicates removed (%)
7.3
Number of peaks
289 (qval < 1E-05)

hg19

Number of total reads
19010646
Reads aligned (%)
97.6
Duplicates removed (%)
7.8
Number of peaks
430 (qval < 1E-05)

Base call quality data from DBCLS SRA