Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GRHL2

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 cells
cell line
MCF7 cells
treatment duration
45 minutes
treatment
Vehicle
antibody
Anti-GRHL2 HPA004820 (Sigma)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA-seq: Hormone-treated cells were homogenized and total RNA was extracted using Qiagen RNAeasy kit RNA-seq: Total RNA was isolated from the experimental cell populations and primary breast tumors using Trizol (Invitrogen). Poly-A selected, stranded RNA-seq libraries were constructed using the TruSeq Stranded mRNA kit (Illumina). ChIP-seq: Hormone-treated were harvested and ChIP was performed as per the directions provided in materials and method of the paper ChIP-seq libraries were prepared using Rubicon kits.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
23419280
Reads aligned (%)
0.0
Duplicates removed (%)
38.8
Number of peaks
0 (qval < 1E-05)

hg38

Number of total reads
23419280
Reads aligned (%)
0.0
Duplicates removed (%)
38.9
Number of peaks
0 (qval < 1E-05)

Base call quality data from DBCLS SRA