GSM3595411: ChIPseq ER MCF7 WT Veh 45min rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7 cells
cell line
MCF7 cells
treatment duration
45 minutes
treatment
Vehicle
antibody
Anti-ER HC-20 (Santa Cruz)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA-seq: Hormone-treated cells were homogenized and total RNA was extracted using Qiagen RNAeasy kit RNA-seq: Total RNA was isolated from the experimental cell populations and primary breast tumors using Trizol (Invitrogen). Poly-A selected, stranded RNA-seq libraries were constructed using the TruSeq Stranded mRNA kit (Illumina). ChIP-seq: Hormone-treated were harvested and ChIP was performed as per the directions provided in materials and method of the paper ChIP-seq libraries were prepared using Rubicon kits.