Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Gonad
Cell type
SVOG-3e
NA
NA

Attributes by original data submitter

Sample

source_name
SVOG3e cells transduced with V5-FOXL2 C134W
cell type
SV40 large T antigen immortalized human granulosa cells
genotype/variation
transduced with V5-FOXL2 C134W lentivirus
induction
12 hours Doxycycline induction
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were trypsinized and collected by spin at 300g for 5 min. Then cell pellets were snap-freezed We performed native chromatin immunoprecipitation (ChIP) as described previously (Lorzadeh et al., 2016). Libraries were constructed using an Agilent Bravo automated liquid handling platform. Illumina sequencing libraries were generated by end repair, 3' A-addition, and Illumina sequencing adaptor ligation.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
56216582
Reads aligned (%)
80.0
Duplicates removed (%)
1.0
Number of peaks
897 (qval < 1E-05)

hg19

Number of total reads
56216582
Reads aligned (%)
79.3
Duplicates removed (%)
1.5
Number of peaks
548 (qval < 1E-05)

Base call quality data from DBCLS SRA