Antigen

Antigen Class

TFs and others

Antigen

CASZ1

Cell type

Cell type Class

Muscle

Cell type

SMS-CTR

NA

NA

Sample

source_name

SMS-CTR cells

cell type

Rhabdomyosarcoma

genotype

stably transfected with CASZ1b, Dox inducible

dox status

Dox

chip antibody

CASZ1 (in collaboration with Rockland, ab8540)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

33717909

Reads aligned (%)

96.8

Duplicates removed (%)

9.7

Number of peaks

22243 (qval < 1E-05)

hg19

Number of total reads

33717909

Reads aligned (%)

96.4

Duplicates removed (%)

10.2

Number of peaks

22154 (qval < 1E-05)