Antigen

Antigen Class

TFs and others

Antigen

TP53

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

Colorectal cancer cells

cell type

HCT116

chip antibody

p53 (CTS 2527)

treatment

5-FU

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed by VATHS Universal DNA Library Prep Kit for Illumina (Vazyme ND604). 

Sequencing Platform

instrument_model

HiSeq X Ten

hg19

Number of total reads

29238764

Reads aligned (%)

89.9

Duplicates removed (%)

50.5

Number of peaks

1277 (qval < 1E-05)

hg38

Number of total reads

29238764

Reads aligned (%)

91.0

Duplicates removed (%)

49.8

Number of peaks

1471 (qval < 1E-05)