Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TFAP2C

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived epidermal cells
NA
NA

Attributes by original data submitter

Sample

source_name
TetO-TFAP2C hESC differentiation at Day7 with Dox
cell type
early epidemal differentiated human emryonic stem cell (hESC)
genotype
TetO-TFAP2C
treatment and stage
early differentiaiton with Dox for 7 days
chip antibody
TFAP2C; Santa Cruz Cat #sc-8977X

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared following the NEBNext protocol (NEBNext ChIP-Seq Library Prep kit Cat #E6240S/L)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
332842071
Reads aligned (%)
2.4
Duplicates removed (%)
97.0
Number of peaks
8121 (qval < 1E-05)

hg38

Number of total reads
332842071
Reads aligned (%)
3.5
Duplicates removed (%)
95.7
Number of peaks
9427 (qval < 1E-05)

Base call quality data from DBCLS SRA