Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived epidermal cells
NA
NA

Attributes by original data submitter

Sample

source_name
TetO-TFAP2C hESC differentiation at Day7 with Dox
cell type
early epidemal differentiated human emryonic stem cell (hESC)
genotype
TetO-TFAP2C
treatment and stage
early differentiaiton with Dox for 7 days
chip antibody
H3K4m1; Abcam Cat #ab8895

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared following the NEBNext protocol (NEBNext ChIP-Seq Library Prep kit Cat #E6240S/L)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
39902359
Reads aligned (%)
98.0
Duplicates removed (%)
65.2
Number of peaks
454 (qval < 1E-05)

hg19

Number of total reads
39902359
Reads aligned (%)
97.7
Duplicates removed (%)
65.6
Number of peaks
465 (qval < 1E-05)

Base call quality data from DBCLS SRA