Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFE2

Cell type

Cell type Class
Blood
Cell type
Erythroid Cells
MeSH Description
The series of cells in the red blood cell lineage at various stages of differentiation.

Attributes by original data submitter

Sample

source_name
Erythroid culture
tissue
Erythroid culture
gender
male
antibody
NF-E2 (sc-477; Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was carried out using ChIP Kit (17-295, Upstate Cell Signalling, now Millipore) following the manufacturers instructions. Briefly 10^7 cells and nuclei were lysed for 10 minutes before chromatin was sheared by sanitation in a Bioruptor (High: 10 min, 30sec on, 30 sec off). Insoluable material was pelleted and supernatant was incubated overnight with the relevant antibody. Antibodies were then conjugated to protein A-agarose and washed. DNA was extracted using phenol-chloroform followed by ethanol precipitation with yeast tRNA carrier. Samples were submitted to Oxford Genomics Center for indexing and sequencing (50 bp Illumina Genome Analyzer II).

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
13803973
Reads aligned (%)
13.0
Duplicates removed (%)
36.8
Number of peaks
3701 (qval < 1E-05)

hg38

Number of total reads
13803973
Reads aligned (%)
13.3
Duplicates removed (%)
36.0
Number of peaks
3591 (qval < 1E-05)

Base call quality data from DBCLS SRA