GSM3579328: H3K4DOX12 S8.merged.bam; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Breast
Cell type
Mammary epithelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
Immortalized human mammary epithelial cells
cell type
Immortalized human mammary epithelial cells
immunoprecipitation
H3K4 trimethylation
treatment
plus doxycycline
antibody
anti-H3K4me ChIP-seq
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslink was performed with disuccinimidyl glutarate (DSG) (Thermo Scientifis) for 45 minutes followed by 30 minutes incubation with formaldehyde 1%. The reaction was blocked with glycine 100 mM. Shearing was performed using Covaris S2 (Covaris, Woburn, MA) for 8 min at maximum intensity. The sonicated chromatin was incubated O/N at 4°C in presence of antibodies as described in (https://elifesciences.org/articles/27706) coupled to A/G sepharose beads (Santa Cruz Biotechnology). Kapa Hyper Prep Kit (Kapa Biosystems, Wilmington, MA) was used for End-repair, A-tailing and ligation of sequence adaptors. Samples were amplified by PCR and the libraries were size-selected in the 200-500 bp range.