Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Lung
Cell type
BEAS-2B
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Beas-2B airway epithelial cells
lentiviral treatment
shCtrl
treatment
TNF+dex
ip antibody
GR-356

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed as previously described (PMID: 23508109) with a 5 minute fixation time in 1% methanol-free formaldehyde and 35 cycles (30-second bursts separated by 30-second incubations in ice water) of sonication. Twelve ug of GR-IA1 (Miles Pufall) or GR-356 (custom-made by Covance) antibody were used for immunoprecipitation. One ng of purified ChIP DNA was used to prepare uniquely barcoded libraries with the Ovation Ultralow Library System from NuGEN. Libraries were pooled and sequenced in duplicate on an Illumina NovaSEQ using 2 x 150 bp paired-end reads.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg19

Number of total reads
37459552
Reads aligned (%)
105.4
Duplicates removed (%)
29.0
Number of peaks
1834 (qval < 1E-05)

hg38

Number of total reads
37459552
Reads aligned (%)
106.5
Duplicates removed (%)
28.8
Number of peaks
2344 (qval < 1E-05)

Base call quality data from DBCLS SRA