Antigen

Antigen Class

TFs and others

Antigen

NR3C1

Cell type

Cell type Class

Lung

Cell type

BEAS-2B

Primary Tissue

Lung

Tissue Diagnosis

Normal

Sample

source_name

Beas-2B airway epithelial cells

lentiviral treatment

shCtrl

treatment

vehicle

ip antibody

GR-356

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) was performed as previously described (PMID: 23508109) with a 5 minute fixation time in 1% methanol-free formaldehyde and 35 cycles (30-second bursts separated by 30-second incubations in ice water) of sonication. Twelve ug of GR-IA1 (Miles Pufall) or GR-356 (custom-made by Covance) antibody were used for immunoprecipitation. One ng of purified ChIP DNA was used to prepare uniquely barcoded libraries with the Ovation Ultralow Library System from NuGEN. Libraries were pooled and sequenced in duplicate on an Illumina NovaSEQ using 2 x 150 bp paired-end reads.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg19

Number of total reads

58134427

Reads aligned (%)

71.7

Duplicates removed (%)

12.1

Number of peaks

474 (qval < 1E-05)

hg38

Number of total reads

58134427

Reads aligned (%)

72.5

Duplicates removed (%)

11.8

Number of peaks

1560 (qval < 1E-05)