GSM3577946: MCF7 ER shCtrl ChipSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7
cell line
MCF7
cell type
Breast cancer cell line
shRNA
Control
treatment
E2
chip antibody
ERalpha (Santa Cruz, sc-7207)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody or by streptavidin magnet beads pull-down. The extracted ChIP DNA was ligated to specific adaptors of KAPA Hyper Prep system (KK8504), according to the manufacturer's instructions.