Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D
cell line
T47D
cell type
Breast cancer cell line
treatment
E2
chip antibody
ERalpha (Santa Cruz, sc-7207)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody or by streptavidin magnet beads pull-down. The extracted ChIP DNA was ligated to specific adaptors of KAPA Hyper Prep system (KK8504), according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg19

Number of total reads
23748956
Reads aligned (%)
77.4
Duplicates removed (%)
18.3
Number of peaks
5636 (qval < 1E-05)

hg38

Number of total reads
23748956
Reads aligned (%)
79.4
Duplicates removed (%)
17.1
Number of peaks
5665 (qval < 1E-05)

Base call quality data from DBCLS SRA