Antigen

Antigen Class

Histone

Antigen

H4

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

U2OS osteosarcoma cells

cell line

U2OS

cell type

Osteosarcoma cells

genotype/variation

RB1+/+

chip antibody

H4 (clone 62-141-13, EMD Millipore)

Sequenced DNA Library

library_name

ERROR: cannot retrieve data from NCBI: too many requests

library_strategy

ERROR: cannot retrieve data from NCBI: too many requests

library_source

ERROR: cannot retrieve data from NCBI: too many requests

library_selection

ERROR: cannot retrieve data from NCBI: too many requests

library_construction_protocol

ERROR: cannot retrieve data from NCBI: too many requests

Sequencing Platform

instrument_model

ERROR: cannot retrieve data from NCBI: too many requests

cycle_sequence

ERROR: cannot retrieve data from NCBI: too many requests

cycle_count

ERROR: cannot retrieve data from NCBI: too many requests

flow_sequence

ERROR: cannot retrieve data from NCBI: too many requests

flow_count

ERROR: cannot retrieve data from NCBI: too many requests

key_sequence

ERROR: cannot retrieve data from NCBI: too many requests

hg38

Number of total reads

19875825

Reads aligned (%)

90.2

Duplicates removed (%)

3.8

Number of peaks

1099 (qval < 1E-05)

hg19

Number of total reads

19875825

Reads aligned (%)

89.2

Duplicates removed (%)

6.0

Number of peaks

915 (qval < 1E-05)