GSM3568778: U937 shKDM6A 2% Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
U-937
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma Diffuse Large B Cell
Attributes by original data submitter
Sample
source_name
U937_shKDM6A_2% Input
cell line
U937
cell type
Pro-monocytic, human myeloid leukemia cell line
genotype/variation
shKDM6A
chip antibody
2% Input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were precipitated with antibody. ChIP-seq library preparation was performed using TruSeqChIP Sample Prep Kit (Illumina) according to the manufacturer's instructions.10ng of input ChIP-enriched DNA was used for ChIP-seq library preparation. Final libraries were checked using High Sensitivity chips in 2100 Bioanalyzer (Agilent). Average fragment size of final libraries was found to be 280 ± 8bp. Paired-end sequencing (2 x 100 bp) of these libraries were performed in HiSeq-2500 (Illumina) according to the manufacturer's protocols.